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Dynal 免疫磁珠:快捷纯化人Regulatory T Cells
2003/10/27 10:48:55  飞捷生物
 
Isolate Human Regulatory T Cells the Easy Way!
 
If you are looking for a high yield and purity of human regulatory T cells, with the flexibility to adapt your protocol, try the new Dynal® CD4+CD25+ Treg Kit (Prod.No 113.23) for:
  • High yield & purity of Treg cells
  • Improve suppressive effect by depleting CD45RA+ cells (optional)
  • Bead-free cells avoid potential cell activation
  • Isolated cells are suitable for all downstream applications
  • Isolate from MNC (up to 1 x 109 total MNC) per kit



You can adapt your protocol depending on your requirements :

Choice 1 – do you want CD25+CD45RA+ cells in your sample?
CD45 mAb is supplied separately in the kit so you decide if you add it to the antibody mix:

+ CD45 mAb : remove these cells for enriched Treg cells and increased T cell suppression.
- CD45 mAb : keep all CD4+CD25+ T cells for your downstream application

Choice 2 – do you want bead-free cells ?

Bead-free cells – detach the beads so Treg cells can be used in flow cytometry, suppressive assays or any other downstream assays.
Cells-with-beads – for molecular studies.

This easy, reliable method uses a standard tube with a magnet, with no need for columns. You just need to decide what to do with the isolated cells!
 
 
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